Antibodies
Anti-GAPDH Rabbit Polyclonal Antibody
Catalog No.: LRC-ORB10805
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Full length human GAPDH recombinant protein |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification Method | Affinity purification |
| Formulation | Liquid |
| Storage Buffer | PBS, pH 7.4, 50% glycerol, 0.02% sodium azide |
| Storage Temperature | -20°C for 12 months |
| Concentration | 1 mg/mL |
| Tested Applications | WB, IHC-P, IF, FC |
| Recommended Dilution | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 |
Description
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is a key enzyme in glycolysis. It is constitutively expressed at high levels in most tissues and is commonly used as a loading control for Western blotting. This antibody recognizes GAPDH protein in human, mouse, rat, and monkey samples.
Technical Specifications
| Catalog Number | LRC-ORB10805 |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Full length human GAPDH recombinant protein |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification Method | Affinity purification |
| Formulation | Liquid |
| Storage Buffer | PBS, pH 7.4, 50% glycerol, 0.02% sodium azide |
| Storage Temperature | -20°C for 12 months |
| Concentration | 1 mg/mL |
| Tested Applications | WB, IHC-P, IF, FC |
| Recommended Dilution | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 |
Product Overview
GAPDH is a 36 kDa enzyme that catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate. It is widely used as a housekeeping gene and protein loading control due to its stable expression across most tissue types and experimental conditions.
Applications
| Application | Dilution | Species |
|---|---|---|
| WB | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
| IHC-P | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
| IF | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
| FC | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
Product Images
Western blot analysis of GAPDH in HeLa cell lysate.
Immunofluorescence staining of GAPDH in HeLa cells.
Western blot analysis of GAPDH in HeLa cell lysate.
Immunofluorescence staining of GAPDH in HeLa cells.
Western blot analysis of GAPDH in HeLa cell lysate.
Immunofluorescence staining of GAPDH in HeLa cells.
Documents
Protocol
Western Blot Protocol:
1. Load 20-30 μg protein per lane
2. Transfer to PVDF membrane
3. Block with 5% non-fat milk in TBST, 1hr
4. Incubate primary antibody 1:5000 overnight at 4°C
5. Wash 3x with TBST
6. Incubate HRP-secondary 1:5000, 1hr
7. Wash and detect with ECL
1. Load 20-30 μg protein per lane
2. Transfer to PVDF membrane
3. Block with 5% non-fat milk in TBST, 1hr
4. Incubate primary antibody 1:5000 overnight at 4°C
5. Wash 3x with TBST
6. Incubate HRP-secondary 1:5000, 1hr
7. Wash and detect with ECL
